The track begins on a dirt road. There is a curving wooden bridge when the player reaches there and there are item boxes for the player to take. After the item boxes, there is a single red mushroom that the player can use a trick on to gain momentum. Before this mushroom, other mushrooms can be seen. In between the road to the bridge and mushroom is an abyss the player can fall into.
After the mushroom is a left turn in which the player has a choice to take the longer, but safe path or the more dangerous, but short path with the mushrooms on them. The path the A.I. will usually take is the mushroom path, which has several red mushrooms and some green mushrooms. Before the mushrooms is a ramp with a boost panel on it so that the player can perform a trick and boost on the red mushrooms. The alternate path is a curving path that first swerves to the left and then to the right, back to the normal track. It has item boxes and boost panels there to help the player or to throw the player off course.
After the mushrooms/alternate path, there is an entrance to a cave. In the cave, the road turns left and into an abyss with mushrooms all over. The player also has two paths to take, and both involve the use of the mushrooms in the track. One path on the right is normal while the one on the left will take the player to a greater height. Both paths have green mushrooms, red mushrooms, and item boxes for the player to use and both paths won't have much of a time difference between them.
After the mushrooms is the end of the cave and the last turn, which is a U-turn left. Goombas are on this part of the track, either helping the player with the coughing up of their mushrooms or hindering the player by running into them. On the extreme left is an abyss for players not careful who are using a mushroom to cut through the grass in this part of the track. After the Goombas is the finish line and another lap.
In Mushroom Gorge, there is a shortcut that will allow the player to speed past the regular curving route and get a boost, although it requires a Mushroom, and is recommended for Time Trials. After starting the race with a turbo boost, the player should head to the left. After the player has headed left, they'll see that there's no fence and that there's a crevice. As soon as the player is a few lengths away from the crevice, use a Mushroom to get a boost. The player will be flung onto a large Mushroom. Perform a trick, bounce on the other giant Mushroom, perform a trick, and the player should land back on the pathway.
Also, by the last U-turn, if the player cuts across the grassy patch with some sort of speed boost and doesn't fall into the crevice, there is a time gain of a second or two.